Интерфазный анализ Х-анеуплоидии методом флюоресцентной гибридизации in situ в разных тканях здоровых лиц

The frequency of spontaneous X-chromosome hypo- and hyperploidy was studied in interphase nuclei of cultured and uncultured blood lymphocytes and oral epithelium of 21 healthy individuals by means of fluorescence in situ hybridization of the centromere-specific DNA probe. In women, the frequencies of haploid epithelial cells and cultured and uncultured lymphocytes were 0.84%, 1.59%, and 0.73%, respectively, while the frequency of hyperploid cells was 0.29%, 0.28%, and 0.12% respectively. In men, the X-chromosome spontaneous hyperploidy of epithelial cells, culturesd and uncultured lymphocytes was 0.14%, 0.57%, and 0.11% respectively. The upper frequency limit of the spontaneous aneuploidy of various cell types was determined for the purpose of being used for the diagnosis of X-mono- and trisomy mosaicism in women and the disomy in men. In the latter, the frequency of hypoploidy in the cultured lymphocytes was significantly higher than in uncultured lymphocytes. Under conditions of intense cell proliferation in FHA-stimulated cultures, more severe disturbances in chromosome segregation are proposed to occur than in resting cells.Читать в источнике